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2 µ m dapi fluorescent probe  (Thermo Fisher)


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  • 90

    Structured Review

    Thermo Fisher 2 µ m dapi fluorescent probe
    2 µ M Dapi Fluorescent Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+%C2%B5+m+dapi+fluorescent+probe/pmc11694080-145-17-21
    Average 90 stars, based on 1 article reviews
    2 µ m dapi fluorescent probe - by Bioz Stars, 2026-09
    90/100 stars

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    Cell Characterization:

    Article Title: Immunometabolic Effect of Nitric Oxide on Human Macrophages Challenged With the SARS‐CoV2‐Induced Cytokine Storm. A Fluxomic Approach
    Article Snippet: Then, the buffy coat layer was collected in a new tube, washed twice with PBS, and centrifuged for 5 min at 300 g . Residual erythrocytes were lysed by incubating with red blood lysis buffer (420 302, Biolegend) for 10 min and then washing twice with PBS. .. Cell count and viability were addressed in a flow cytometer (Cytoflex‐S, Becton Dickinson) using 2 μ m DAPI fluorescent probe (D1306, Invitrogen). .. After that, PBMC were centrifuged for 5 min at 300 g and resuspended in serum‐free DMEM (41966‐029, Gibco) medium with 100 U mL −1 penicillin and streptomycin (15140–122, Gibco), and then seeded at 1–2×10 6 cells well −1 in 6‐well culture plates or 10×10 6 cells in 100 mm plates (353 046, Falcon).

    Flow Cytometry:

    Article Title: Immunometabolic Effect of Nitric Oxide on Human Macrophages Challenged With the SARS‐CoV2‐Induced Cytokine Storm. A Fluxomic Approach
    Article Snippet: Then, the buffy coat layer was collected in a new tube, washed twice with PBS, and centrifuged for 5 min at 300 g . Residual erythrocytes were lysed by incubating with red blood lysis buffer (420 302, Biolegend) for 10 min and then washing twice with PBS. .. Cell count and viability were addressed in a flow cytometer (Cytoflex‐S, Becton Dickinson) using 2 μ m DAPI fluorescent probe (D1306, Invitrogen). .. After that, PBMC were centrifuged for 5 min at 300 g and resuspended in serum‐free DMEM (41966‐029, Gibco) medium with 100 U mL −1 penicillin and streptomycin (15140–122, Gibco), and then seeded at 1–2×10 6 cells well −1 in 6‐well culture plates or 10×10 6 cells in 100 mm plates (353 046, Falcon).



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    90
    Thermo Fisher 2 µ m dapi fluorescent probe
    2 µ M Dapi Fluorescent Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+%C2%B5+m+dapi+fluorescent+probe/pmc11694080-145-17-21
    Average 90 stars, based on 1 article reviews
    2 µ m dapi fluorescent probe - by Bioz Stars, 2026-09
    90/100 stars
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